Karyotyping is a multistep process that transforms live cells into examinable digital images of chromosomes.
The process begins with samples aseptically collected from the animal to be analyzed.
In the laboratory, the cells are placed in nutritive culture medium
The culture is placed in an incubator at 37°C to allow growth.
After 3 days, cells are removed from the culture, washed, then fixed in an acid methanol solution to preserve the DNA.
The fixed cells are then dropped onto slides, stained.
Slides are examined under a microscope.
The chromosomes are analyzed and the karyotype made according to international guidelines.